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How Does Exogenous Recombination Work?

exogenous recombination

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#1 Anchovyforestbane

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Posted 07 March 2020 - 02:54 PM

How in the world does exogenous recombination (cellular transformation) work? Nothing I've read seems to specify how it happens.



#2 VictorMedvil

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Posted 07 March 2020 - 03:55 PM

How in the world does exogenous recombination (cellular transformation) work? Nothing I've read seems to specify how it happens.

It can happen in various ways, any method that makes foreign DNA get included into a cell will transform the cell. Generally, I use Viral Vectors("Expression Vector") however one could use any number of methods to insert foreign DNA into the cell.

Read this link for more details = https://www2.le.ac.u...inanttechniques

 

 

Here is a flow diagram for exogenous recombination using Vectors.

3-s2-0-B9780128046593000191-f19-03-97801

 

Also there are techniques like protoplast fusion which can fuse the DNA of two or more living cells together chemically. Link = https://link.springe...-642-81784-7_13

 

Flow Diagram for Protoplast fusion by Polyethylene Gycol(PEG)

clip-image0077.jpg

 

That's how I created this Plant/Fungus/Bacteria organism was via Protoplast fusion between 45 different species of organisms, which I named "Metamorphic Gel"

 

20190125-090933.jpg

 

This is what happened when I then merged a Human T-cell(White Blood Cell) with the Metamorphic Gel making T-Cell Metamorphic Gel 46 using protoplast fusion.

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 So, basically there are many ways to do exogenous recombination just depends on which method you are comfortable with using.


Edited by VictorMedvil, 07 March 2020 - 08:52 PM.

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