joho Posted January 13, 2008 Report Share Posted January 13, 2008 Am still revising but have confused myself somewhat over whether the above are the same thing or two different things. In my notes I have: normal phase silica gel with Si-O-SiOC18H37 groups and SiOH active sites for interaction with analytes and called that ODS1. reverse phase HPLC as being chemically modified and having Si-OSiOCH3 groups on the active sites and called that ODS2. I then have notes describing end-capping as the reaction of organochlorosilanes with silanol groups and an equation of SiOH + C18H37SiCl3 being silylated and hydrolysed to Si-OSiC18H37 (+ 2HCl) but thats the same as the groups on the NPHPLC. If the active sites on RP HPLC are already modified how can this end-capping occur? Am confusing myself even more and can't even get the right words for my questions to make sense!:) Can anyone help again! Thank you. Edit: Found the answer but can't delete this post - sorry! Will leave it in case anyone wants to answer anyway! Quote Link to comment Share on other sites More sharing options...
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