annle132 Posted April 12, 2007 Report Share Posted April 12, 2007 Can anyone help me? :confused:I am trying to purify a protein out of a nuclear extract. I know absolutely nothing about this protein (transcription factor), except which DNA sequence it binds to. I try to follow the purification through a gel shift (EMSA) assay and have heard that you can calculate the dissociation constant (Kd) from gel shifts.Do anyone know how :hyper: I would be thankful for all your help! /Anna Imagination is more important than knowledge...Albert Einstein Quote Link to comment Share on other sites More sharing options...
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