Well today I did some research on Huntington's disease and I have come up with a plan of attack on the disease. It seems the disease is caused from a Prion which is a mutated gene of naturally occurring Huntingtin gene which has extra repeats of "CAG" which causes the misfold of the protein with this knowledge I have decided to create a Viral Vector to remove the repeats. This treatment would need to be used in combination with genome sequencing the measure the actual amount of "CAG" repeats are present in the protein to make a custom vector based upon the number of them that cleaves and removes that section of DNA from every neuron.
The Actual Vector would need to be a Retroviral Vector with Rabies glycoprotein pseudotyping to target neurons also this would require a custom Cas9 and guide RNA insert which will repeated based upon how many "CAG" extra sequences there are in the gene greater than 35 repeats. The Gene map for the Vector and insert is below.
Edited by VictorMedvil, 05 June 2020 - 09:11 PM.